TrumpLyftAlles | 13 points
Quantitative proteomics reveals a broad-spectrum antiviral property of ivermectin, benefiting for COVID-19 treatment (China 2020-09-22)I love how it's going to take a full year but eventually doctors/scientists in the USA will finally catch onto ivermectin
I wonder how many of those quarter million lives could have been saved in the meanwhile if it was readily available, maybe not OTC but a prescription without hesitation by family doctors.
Could it have saved 10,000 lives? 25,000? 50,000? It's a shame how stubborn (and stupid) our country can be at times.
My guess is that early hcq cocktail treatment would have cut deaths by a minimum of 25%, if not more (50 lives). Ivermectin cocktail might be able to cut it down to nearly zero (of course I’m not sure). Considering the safety profile of these drugs, there is no way to defend state level interventions between doctors and patients. In PA, docs cannot prescribe either drug - I know from personal experience.
[-] Alexanderandjeff | 1 points
They'll find ways to discredit this drug since it makes no money for them and appears to be a ubiquitous anti viral killer and income killer for pharmaceutical companies and doctors. Maybe Clorine Dioxide will be next and compliment Ivermectin....The two used in tandem should kill just about every pathogen known or even unknown .
It's been an eye-opening year for me. I knew people were stupid but not that stupid, and in such vast numbers.
There are times when RCTs are appropriate - namely for researching treatments into chronic health issues... but aren't we being way too cautious here? By the time the US gets it head out of its ass and completes studies, I feel the same - we're going to have hundreds of thousands if not millions of avoidable deaths.
Then there's the "concentration too low" argument - it's a head in the sand, I know everything, there's only one possible way this could work, kind of stupid argument - the kind of argument that this paper counters. We don't know for sure if it works, or how it works, but it does seem to work and it's a very safe drug, so why is the US so overcautious?
I don't see how the HCQ debacle is even a close comparison to this situation. Having taken HCQ in the past and dealing with its horrible side effects, which are well known, I just don't see the comparison to ivermectin which has a well established safety profile.
I might keep tubes of this shit around to use before air travel in the future. I always come back from work trips with a cold, but maybe a squirt of ivermectin would make that less of a problem. We'll see.
[-] TrumpLyftAlles | 4 points | Sep 24 2020 04:56:51
TL;DR from a brief glance: there are lots of ways ivermectin can interact with the coronavirus. These are more mechanisms they may underlie ivermectin's therapeutic effecti.
The article looks like a good detailed write-up of ivermectin ressearch.
I've never heard of quantitative proteomics. From wikipedia:
Quantitative proteomics is an analytical chemistry technique for determining the amount of proteins in a sample.[1][2][3] The methods for protein identification are identical to those used in general (i.e. qualitative) proteomics, but include quantification as an additional dimension. Rather than just providing lists of proteins identified in a certain sample, quantitative proteomics yields information about the physiological differences between two biological samples. For example, this approach can be used to compare samples from healthy and diseased patients. Quantitative proteomics is mainly performed by two-dimensional gel electrophoresis (2-DE) or mass spectrometry (MS). However, a recent developed method of quantitative dot blot (QDB) analysis is able to measure both the absolute and relative quantity of an individual proteins in the sample in high throughput format, thus open a new direction for proteomic research. In contrast to 2-DE, which requires MS for the downstream protein identification, MS technology can identify and quantify the changes.
Applications
Work flow of the Quantification of the physiological differences in α and β cells in mice using computer prediction (A) and SILAC isotope-label quantification(B). (C) is the candidate list of kinases that indicate physiological differences in α and β cells.[19] Biomedical applications Quantitative proteomics has distinct applications in the medical field. Especially in the fields of drug and biomarker discovery. LC-MS/MS techniques have started to over take more traditional methods like the western blot and ELISA due to the cumbersome nature of labeling different and separating proteins using these methods and the more global analysis of protein quantification. Mass spectrometry methods are more sensitive to difference in protein structure like post-translational modification and thus can quantify differing modifications to proteins. Quantitative proteomics can circumvent these issues, only needing sequence information to be performed. Disadvantages, however, in sensitivity and analysis time must be kept in consideration.[20]
Drug discovery Quantitative proteomics has the largest applications in the protein target identification, protein target validation, and toxicity profiling of drug discovery.[21] Drug discovery has been used to investigate protein-protein interaction and, more recently, drug-small molecule interactions. Thus, it has shown great promise in monitoring side-effects of small drug-like molecules and understanding the efficacy and therapeutic effect of one drug target over another.[22][23] One of the more typical methodologies for absolute protein quantification in drug discovery is the use of LC-MS/MS with multiple reaction monitoring (MRM). The mass spectrometry is typically done by a triple quadrupole MS.[21]
OK, it's another way. Scientists are awesome.
This study:
Abstract
Viruses such as human cytomegalovirus (HCMV), human papillomavirus (HPV), Epstein–Barr virus (EBV), human immunodeficiency virus (HIV), and coronavirus (severe acute respiratory syndrome coronavirus 2 [SARS‐CoV‐2]) represent a great burden to human health worldwide. FDA‐approved anti‐parasite drug ivermectin is also an antibacterial, antiviral, and anticancer agent, which offers more potentiality to improve global public health, and it can effectively inhibit the replication of SARS‐CoV‐2 in vitro.
This study sought to identify ivermectin‐related virus infection pathway alterations in human ovarian cancer cells. Stable isotope labeling by amino acids in cell culture (SILAC) quantitative proteomics was used to analyze human ovarian cancer cells TOV‐21G treated with and without ivermectin (20 μmol/L) for 24 h, which identified 4447 ivermectin‐related proteins in ovarian cancer cells. Pathway network analysis revealed four statistically significant antiviral pathways, including HCMV, HPV, EBV, and HIV1 infection pathways.
Interestingly, compared with the reported 284 SARS‐CoV‐2/COVID‐19‐related genes from GencLip3, we identified 52 SARS‐CoV‐2/COVID‐19‐related protein alterations when treated with and without ivermectin. Protein–protein network (PPI) was constructed based on the interactions between 284 SARS‐CoV‐2/COVID‐19‐related genes and between 52 SARS‐CoV‐2/COVID‐19‐related proteins regulated by ivermectin. Molecular complex detection analysis of PPI network identified three hub modules, including cytokines and growth factor family, MAP kinase and G‐protein family, and HLA class proteins. Gene Ontology analysis revealed 10 statistically significant cellular components, 13 molecular functions, and 11 biological processes.
These findings demonstrate the broad‐spectrum antiviral property of ivermectin benefiting for COVID‐19 treatment in the context of predictive, preventive, and personalized medicine in virus‐related diseases.
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