Ivermectin is a specific inhibitor of importin α/β-mediated nuclear import able to inhibit replication of HIV-1 and dengue virus (Australia 2012-04-12)
More verification of ivermectin's importin α/β mechanism, esp. "we characterized in detail the nuclear transport inhibitory properties of ivermectin, demonstrating that it is a broad-spectrum inhibitor of importin α/β nuclear import".
Abstract
The movement of proteins between the cytoplasm and nucleus mediated by the importin superfamily of proteins is essential to many cellular processes, including differentiation and development, and is critical to disease states such as viral disease and oncogenesis. We recently developed a high-throughput screen to identify specific and general inhibitors of protein nuclear import, from which ivermectin was identified as a potential inhibitor of importin α/β-mediated transport. In the present study, we characterized in detail the nuclear transport inhibitory properties of ivermectin, demonstrating that it is a broad-spectrum inhibitor of importin α/β nuclear import, with no effect on a range of other nuclear import pathways, including that mediated by importin β1 alone. Importantly, we establish for the first time that ivermectin has potent antiviral activity towards both HIV-1 and dengue virus, both of which are strongly reliant on importin α/β nuclear import, with respect to the HIV-1 integrase and NS5 (non-structural protein 5) polymerase proteins respectively. Ivermectin would appear to be an invaluable tool for the study of protein nuclear import, as well as the basis for future development of antiviral agents.
This and the role of importin α/β explained at length, with illustrations, here. It is theorized that ivermectin prevents the import of covid-19 proteins into the nucleus that would otherwise disable the cell's immune response.
Care to explain why that's relevant? Instead of just dropping a bomb, why don't you courteously explain you're talking about? During your explanation, don't bring up viral replication because we're aware of the fact that SARS-CoV-2 doesn't replicate in the nucleus.
My post mentioned proteins entering the nucleus. Are you equating viral RNA to proteins?
there is no known proof of viral RNA entering the nucleus without reverse transcriptase to cause anything.
Is there proof to the contrary?
Theories are theories, they're all theoretical.
Can you provide a link about a paper that says "viral RNA entering the nucleus without reverse transcriptase" that is controversial? That describes the science yay and nay? THAT would be useful. Blanket statements are not, especially when they have no apparent connection to the thread.
You wrote:
Coronavirus is not a retrovirus.
I still have no idea why that's relevant. Lay it out for me, please, like an ELI5.
Here is a facetious remark, trying to get into the spirit of your posts in this thread:
You don't really need to change your oil as often as the owner's manual says.
Your citations mention Dengue and HIV. Be diligent.
OK, now I understand why "Coronavirus is not a retrovirus. Can we get this through our thick heads yet?" is a scientifically-valid statement that educates the sub.
[-] TrumpLyftAlles | 2 points | Aug 28 2020 08:59:48
More verification of ivermectin's importin α/β mechanism, esp. "we characterized in detail the nuclear transport inhibitory properties of ivermectin, demonstrating that it is a broad-spectrum inhibitor of importin α/β nuclear import".
Abstract
The movement of proteins between the cytoplasm and nucleus mediated by the importin superfamily of proteins is essential to many cellular processes, including differentiation and development, and is critical to disease states such as viral disease and oncogenesis. We recently developed a high-throughput screen to identify specific and general inhibitors of protein nuclear import, from which ivermectin was identified as a potential inhibitor of importin α/β-mediated transport. In the present study, we characterized in detail the nuclear transport inhibitory properties of ivermectin, demonstrating that it is a broad-spectrum inhibitor of importin α/β nuclear import, with no effect on a range of other nuclear import pathways, including that mediated by importin β1 alone. Importantly, we establish for the first time that ivermectin has potent antiviral activity towards both HIV-1 and dengue virus, both of which are strongly reliant on importin α/β nuclear import, with respect to the HIV-1 integrase and NS5 (non-structural protein 5) polymerase proteins respectively. Ivermectin would appear to be an invaluable tool for the study of protein nuclear import, as well as the basis for future development of antiviral agents.
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[-] TrumpLyftAlles | 1 points | Aug 28 2020 09:04:16
protein nuclear import
This and the role of importin α/β explained at length, with illustrations, here. It is theorized that ivermectin prevents the import of covid-19 proteins into the nucleus that would otherwise disable the cell's immune response.
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[-] No-Sandwich-777 | 2 points | Aug 29 2020 03:08:26
Coronavirus is not a retrovirus. Can we get this through our thick heads yet?
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[-] TrumpLyftAlles | 1 points | Aug 29 2020 03:15:11
Coronavirus is not a retrovirus.
Care to explain why that's relevant? Instead of just dropping a bomb, why don't you courteously explain you're talking about? During your explanation, don't bring up viral replication because we're aware of the fact that SARS-CoV-2 doesn't replicate in the nucleus.
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[-] No-Sandwich-777 | 1 points | Aug 29 2020 03:54:13
Your citations mention Dengue and HIV. Be diligent.
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[-] No-Sandwich-777 | 1 points | Aug 29 2020 04:00:30
As far as I know, there is no known proof of viral RNA entering the nucleus without reverse transcriptase to cause anything. Purely hypothetical.
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[-] TrumpLyftAlles | 1 points | Aug 29 2020 04:14:20
proteins
My post mentioned proteins entering the nucleus. Are you equating viral RNA to proteins?
there is no known proof of viral RNA entering the nucleus without reverse transcriptase to cause anything.
Is there proof to the contrary?
Theories are theories, they're all theoretical.
Can you provide a link about a paper that says "viral RNA entering the nucleus without reverse transcriptase" that is controversial? That describes the science yay and nay? THAT would be useful. Blanket statements are not, especially when they have no apparent connection to the thread.
You wrote:
Coronavirus is not a retrovirus.
I still have no idea why that's relevant. Lay it out for me, please, like an ELI5.
Here is a facetious remark, trying to get into the spirit of your posts in this thread:
You don't really need to change your oil as often as the owner's manual says.
permalink
[-] No-Sandwich-777 | 1 points | Aug 29 2020 05:50:49
https://onlinelibrary.wiley.com/doi/full/10.1111/j.1600-0854.2007.00579.x
Read
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[-] TrumpLyftAlles | 1 points | Aug 29 2020 04:21:41
Your citations mention Dengue and HIV. Be diligent.
OK, now I understand why "Coronavirus is not a retrovirus. Can we get this through our thick heads yet?" is a scientifically-valid statement that educates the sub.
I'm being sarcastic.
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